Keep a list of antibodies. Arrange by color and antigen. Always check against the list before buying.
Put popular target on popular channels
Have sets of panels
Put independent targets on rare channels or biotinylated.
Backup on key antibodies. Check level before key experiment.
Optimize. Dose.
Transition from non-active to active
Chinese culture.
My journey in website building started with Case Western Reserve University. When I initiated a pbwiki site to share information about Cleveland and Case Western. It didn’t get much attention. However, Zhong Wang, the former president was very encouraging. Then I get more interested in making websites. Besides ChinaBlog.cc, I also started these website:
Hanfu.info: A news translation website about hanfu costumes in China. I followed Chinese news about hanfu and translate them into English. Hope I can bridge the language gap between east and western culture. The website eventually failed and I lost my enthusiasm. A few lessons learned: 1) I overestimated the needs for such information outside the Chinese culture. 2) I am not a hanfu expert or cult; I am not a big translation fan either 3) News translation is really of little value; and even those news themselves had little value.
eastseal.com/lishengshi.com: I originally planned to build a website devoted to Chinese seal carving. Met similar problems as hanfu. The need is low, not to mention in English; I am not really an expert, I cannot contribute that much. I ended up making an inactive website for my master LiShengShi.
pkubio.org: I felt we needed a website for our reunion. So I created this website. It’s doing what it supposed to do. I don’t think it will grow much as I originally speculated. We are still a loose organization.
I have long been interested in finance and investing since the craze in 2007
I bought my Trek 7000 happily in August 2006, when summer is partly over. I rode back from the B&K bike store in Mayfield. Then I decided to start a bike club, which I always dreamed to join in college. Over the years, we had a few close members: Feng Xue, Qingjiang Li, Chao Fang. And a few pop-ups: Wang Yang, Wenqian Hu, Mingming Zhang. Lesson learned: those who are interested will always try to make it; those who are not interested will not come anyway.
Haven’t written any short poems for years (longer ones either). Inspired by lyrics of the Ballad of Serenity (Firefly TV series), I wrote these sentences to all the people who “could only be friends”.
Be Her Friend
2011.10.12
Give your love, give your heart,
Give your attention that pushes her far;
She doesn’t care, she smiles in silence,
She says you are one of her friends.
Take her out, to the dates,
Tell her your deep secrets;
From the beginning, to the end,
She never thinks of you more than a friend.
Plenty other fish in the ocean,
Moving forward you pretend.
But she’d better just be one of your friends.
Allspice@MITBBS posted a reply which is pretty funny:
Save your love, save your heart,
Save your attention that pushes me away,
I don’t care, I only smile,
What I can tell is you are one of my friends.
Take me out, to the dates,
Tell me your deep secrets;
From the beginning, to the end,
You are anyone but more than a friend.
Plenty other fish in the ocean.
Moving forward you’d better be,
I enjoy just being one of your friends.
Lindsay Campbell 网络捧出来的美女，演员+Web Journalist， 在CBS做过主播，给Lowe’s Hallmark Miller等拍过广告。下面这三段是去年volvo的广告。觉得挺不错的 分享一下
http://www.youtube.com/view_play_list?p=C3F4053A7BBE2E4D
Update #2: lavie.wordpress.com上的十多篇帖子也转了过来 这下齐活了 噢也
Update 04/27/2009: 昨晚使用fishy的同学的live space mover把live space上四年全部181篇帖子以及所有评论全部转了过来。发帖时间也全部保留，非常完美。大家可以点导航栏的archives查看。
Welcome to WordPress! Welcome to laviex.com!
laviex.com/blog正式上线第一帖！
M$ live space上的老帖子，目前只通过RSS导入了最近的30帖，而且没有评论。这里一个加拿大华人做了一个小工具可以导出所有帖子及评论，但是目前还有bug，不能正确处理发帖时间。所以我打算等这个bug修复以后把所有老帖都转过来！开通四年来一共 181帖！
转移到这里可能会失去不少的读者，特别是MSN上熟识的朋友；但是为了脱离M$的桎梏，只能忍痛了！
你可以通过RSS订阅（侧边拦上部的图标）；也可以通过facebook和twitter和我联系
facebook profile: http://profile.to/yliu
twitter: @6yi
以前曾经告别过一次(2006?) 转到blogger 或 wordpress.com但均以国内无法访问而告终
而今迈步从头越！俺决定再一次尝试，原因如下：
0. live space太烂了 sucks!
1. hotmail开通pop3了 俺决定告别hotmail
2. microblog流行 喊一嗓子的事情不用写blog了 直接facebook or twitter。而 blog则用来写稍有保留价值的内容
3. 不想继续被blog provider所限；最近积累一些自己架站的经验
拥抱自由 Freeeeeeeeeeeeeeeeedom!
新站建起来之后 这里会留个链接 作为最后一文！
希望能成功告别live space!
最近全球facebook用户疯狂流行quiz
我也来赶时髦 创建了一个 how much do you know about China? 链接如下
http://apps.facebook.com/how-much-do–iiahfc
欢迎大家娱乐娱乐
最近迷浙江卫视的朱丹，赫然意识到现在荧屏上的她是与自己同时代的人。以前总觉得这些社会的精英人物是前辈级的人物，现在看到朱丹和华少自如得体幽默地主持浙江卫视的几大娱乐节目，真的有点唏嘘，觉得自己应该加油。以前刘翔牛的时候，还有去年金晶的成熟表现，也只是觉得他们是代表八零后发出一些声音。而今看到朱丹，才真正有了同龄人占据社会主流的感觉。
btw: 俺们八零后——终于掌握话语权了! wahaha 下面该轮到90后挨批了
最近合武高速铁路开通
今年四月起武汉到合肥两小时 到南京3小时 到苏州4小时 到上海5小时…
以后京港全程 沪蓉全程都只要10小时 从武汉去北京、上海、堔港、成都四大城市都只要5个小时火车 想当年我坐公交从武昌火车站到汉口火车站都要两个多小时……武汉以后真的是九省通衢鸟～
这些部分已经实现 全部2012就能实现 …….
btw：我家潜江市也是沪汉蓉高铁一小站（有谁坐过动车组的告诉俺动车组停不停县级小站啊 估计够呛）在武汉到荆州段上 等了一百多年了 家乡终于要有铁路了 而且还是350公里/小时的高速铁路 哇哈哈哈哈哈哈哈
这些年我是摆事实，讲道理，苦口婆心，软硬兼施，却成效甚微?
唉，大家有什么好办法？